Procédé de fixation/décollement sélective/sélectif de cellules, de modélisation cellulaire et de récolte cellulaire au moyen de rayons d'infrarouge proche

Method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays

근적외선에 의한 세포의 선택적 탈착, 패턴 및 수확 방법

Abstract

The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization.
본 발명은 근적외선에 의한 세포의 선택적 탈착, 패턴 및 수확 방법에 관한 것으로, 보다 상세하게는, 근적외선 조사 시 발열특성을 갖는 전도성 고분자 또는 금속산화물을 세포배양용 지지체로 사용하여 효소 처리 없이도 세포를 선택적으로 탈착시킬 수 있고, 상기 지지체는 줄기세포 증식 또는 분화를 촉진하는 효과가 있어 줄기세포 배양 지지체로 사용할 수 있으며, 시간적 공간적 제약 없이 세포 탈착이 가능하여 세포의 패턴화가 가능하다.
La présente invention concerne un procédé de fixation/décollement sélective/sélectif de cellules, de modélisation cellulaire et de récolte cellulaire au moyen de rayons d'infrarouge proche. Plus particulièrement, des polymères ou oxydes métalliques conducteurs, présentant des caractéristiques exothermiques lors de l'irradiation par de la lumière infrarouge proche, sont utilisés comme échafaudage de culture cellulaire, fixant/décollant ainsi sélectivement des cellules sans traitement enzymatique. L'échafaudage présente un effet de favorisation de la prolifération ou de la différenciation des cellules souches et, par conséquent, peut être utilisé comme échafaudage de culture de cellules souches. L'échafaudage permet la fixation/le décollement de cellules sans restrictions temporelles ou spatiales, permettant ainsi la modélisation cellulaire.

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